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Arrayit Corporation
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BioRobotics Ltd
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BioRobotics Ltd
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Compugen Inc
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Arrayit Corporation
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TeleChem International
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Merck KGaA
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Sigma-Genosys
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Image Search Results
Journal: BMC Biotechnology
Article Title: Protein microarray: sensitive and effective immunodetection for drug residues
doi: 10.1186/1472-6750-10-12
Figure Lengend Snippet: Schematics of the ci-ELISA and protein microarray procedures . 5 μg of the artificial antigens (CL or SM 2 ) are coated onto the 96-well plates in (A) ci-ELISA, or 20 ng of the artificial antigens were spotted onto the protein microarray slides in (B) protein microarray. Antibodies against the particular artificial antigens are added together with a range of concentration of the corresponding hapten. As the haptens would compete with the immobilized artificial antigens for binding to the antisera, an increasing concentration of the hapten would result in a decreasing signal. For ci-ELISA, the bound antibodies are visualized by an anti-rabbit-HRP conjugated antibody with the addition of a chromogen (A). For the protein array, the secondary antibodies are conjugated to a fluorescent dye (Cy3), and the signal can be directly measured by a fluorescence microarray scanner (B).
Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on
Techniques: Enzyme-linked Immunosorbent Assay, Microarray, Concentration Assay, Binding Assay, Protein Array, Fluorescence
Journal: BMC Biotechnology
Article Title: Protein microarray: sensitive and effective immunodetection for drug residues
doi: 10.1186/1472-6750-10-12
Figure Lengend Snippet: IC 50 of protein microarray immuntodetection and ci-ELISA . Upper panels: Dose-response curves for (A) CL and (B) SM 2 from ci-ELISA and protein microarray. The log of the hapten concentration (x-axis) was plotted against the percentage of inhibition (y-axis), which is (OD sample /OD control ) × 100%. The control group OD was considered to be the point of 100% activity. The IC 50 was determined by non-linear regression analysis. Lower panels: fluorescent signals from the protein microarray slides for (C) CL and (D) SM 2 . The concentration of the standard solution for each slide is: 1: 0 ng/ml; 2: 1 ng/ml; 3: 5 ng/ml; 4: 10 ng/ml; 5: 20 ng/ml; 6: 100 ng/ml; 7: 1000 ng/ml.
Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on
Techniques: Microarray, Enzyme-linked Immunosorbent Assay, Concentration Assay, Inhibition, Control, Activity Assay
Journal: BMC Biotechnology
Article Title: Protein microarray: sensitive and effective immunodetection for drug residues
doi: 10.1186/1472-6750-10-12
Figure Lengend Snippet: Recoveries of CL from fortified chicken muscle tissues by ci-ELISA and protein microarray
Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on
Techniques: Microarray
Journal: BMC Biotechnology
Article Title: Protein microarray: sensitive and effective immunodetection for drug residues
doi: 10.1186/1472-6750-10-12
Figure Lengend Snippet: Detection of CL from CL-treated chicken muscle tissues by ci-ELISA and protein microarray
Article Snippet: Twenty nanogram of each artificial antigen were spotted onto a 7 × 7 array on
Techniques: Microarray